Unexpected Discovery of Hemoglobin D Iran Families in Punjab, Pakistan by using two different Screening Methods

Background: Hemoglobin D Iran is frequently misdiagnosed as Hb E or Punjab if only one method of screening used. The objective our study was to highlight the importance using two different techniques in diagnosis a hemoglobin variant, case. Hematological parameters heterozygous and compound β/Hb were also compared. Methods: A descriptive carried out on results 52,379 subjects which part thalassemia extended family cascade from 36 districts October 2019-March 2021. Cases run both CE-HPLC (cation exchange-high performance liquid chromatography) CZE (capillary zone electrophoresis). Resulting cases confirmed by ARMS-PCR (Amplification refractory mutation system-polymerase chain reaction). Results: Forty detected 160 initially suspected 126 cases. Diagnosis molecular analysis. Statistical significance found between RBC count, MCV, MCH, F “heterozygous Iran” “compound for β/ Iran”. Conclusion: can be easily missed Punjab, methods are not This maybe reason why remains unreported region. CBC HPLC indices suggestive case Iran. Keywords: Iran, E, Cation exchange High chromatography, Capillary electrophoresis